TRAM P.

Title: Protein Labs

Introduction:

Materials & Methods:

Results:

Figure 1. Bacterial protein expression - control plate with Amp., BL21(DE3), without DNA. There are no signs of bacterial growth.

Figure 2. Bacterial protein expression - experimental plate with Amp., BL21(DE3), DNA (pGEM-gbr22). Light purple colonies.

Figure 3. Bacterial protein expression - fun plate without Amp., organism (BL21(DE3)), DNA (pGEM-gbr22), and bacterial from gloves.

Figure 4. Bacterial protein expression - purple culture in the flask with Amp., BL21(DE3), DNA (pGEM-gbr22).

Figure 5(a&b). Bacterial protein expression - purple cell pellet with Amp., BL21(DE3), DNA (pGEM-gbr22).

Figure 6. Protein purification - elution 1 and elution 2 with purified protein.

Figure 7. Protein purification - Spectrum of elution 1 at 280 nm of gbr22 purified protein.

Figure 8. Protein purification - Spectrum of elution 1 at 574 nm of gbr22 purified protein.

Figure 9. Protein purification - Spectrum of elution 2 at 280 nm of gbr22 purified protein.

Figure 10. Protein purification - Spectrum of elution 2 at 574 nm of gbr22 purified protein.

Figure 11. Protein characterization - gel with bands of cell lysate, soluble fraction, flow through, wash, elution 1, and elution 2.

Discussion:

Conclusions:

References: